2021 Sept 14 - Spatial analysis for visualizing small RNA species implicated in disease and development
Anushka Dikshit, Ph.D.
Sr. Scientist, Supervisor, Applications, Genomics, Advanced Cell Diagnostics
Watch Video:
Outline:

The RNAscope™ in situ hybridization (ISH) technology provides a powerful method to detect gene expression within the spatial and morphological tissue context. The proprietary probe design in combination with advanced signal amplification enables robustly high signal-to-noise and allows for the detection of gene transcripts at the single molecule level with single-cell resolution.

Now, this webcast will present the miRNAscope™ Assay, an advanced in situ hybridization platform that allows for highly specific and sensitive visualization of small RNA species expression with single-cell resolution.

Learning Objectives:
  • The miRNAscope technology and workflow
  • Detecting miRNAs implicated in cancer and distinguishing multiple members from the same family
  • Functional information provided by identifying cell-type specific miRNAs in the brain
  • Visualizing synthetic oligonucleotides such as small interfering RNAs (siRNAs) and anti-sense oligonucleotides (ASOs) that can target specific genes and regulate their expression
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